Single-cell genomics/transcriptomics integrate a series of processes that include the adequate collection and preservation of environmental samples, the physical separation of cells from more or less complex communities followed by cell lysis and genome and/or transcriptome amplification prior to sequencing.
In addition to prokaryotic and eukaryotic microbial cells, UNICELL can sort microbial consortia (e.g. symbioses) and viruses or virus-infected cells.
Still under development, UNICELL proposes a number of standard single-cell ‘omics’ protocols currently working in the facility and offers advice on methods and approaches. For any flow cytometry methodological development on sample analysis and/or cell sorting, please contact us.
Examples of UNICELL activities:
Sorting of planktonic cells from freshwater, sea water and enrichment cultures
Cell sorting based on natural auto-fluorescence (e.g. chlorophyll, Phycoerythrin, Bacteriochlorophyll, F420)
Cell sorting based on fluorescence activated by nucleic acid dyes, cell compartments, etc.
Cell lysis and genomic amplification by the WGA method (Whole Genome Amplification)
Training on the acquisition, analysis and interpretation of the results
Advice to users